Jie Pacelli holds a Ph.D. from Kyushu University (Japan). Dr. Pacelli has extensive experience in maintaining laboratory equipment performance by participating in the definition of operating procedures, establishing quality standards and troubleshooting issues. She actively supports all aspects of lab research at the N.C. Plant Sciences Initiative (N.C. PSI). Dr. Pacelli’s research specializes include the development of analytical methodology for the detection of organic and inorganic components.
- Enzyme Complexes of Ptr4CL and PtrHCT Modulate Co-enzyme A Ligation of Hydroxycinnamic Acids for Monolignol Biosynthesis in Populus trichocarpa , FRONTIERS IN PLANT SCIENCE (2021)
- CAD1 and CCR2 protein complex formation in monolignol biosynthesis in Populus trichocarpa , NEW PHYTOLOGIST (2019)
- Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis , Nature Communications (2018)
- Optoacoustic imaging identifies ovarian cancer using a microenvironment targeted theranostic wormhole mesoporous silica nanoparticle , BIOMATERIALS (2018)
- Phenolation to Improve Lignin Reactivity toward Thermosets Application , ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2018)
- A cell wall-bound anionic peroxidase, PtrPO21, is involved in lignin polymerization in Populus trichocarpa , Tree Genetics & Genomes (2016)
- Structural Characterization of Pine Kraft Lignin: BioChoice Lignin vs Indulin AT , JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY (2016)
- 4-Coumaroyl and Caffeoyl Shikimic Acids Inhibit 4-Coumaric Acid: Coenzyme A Ligases and Modulate Metabolic Flux for 3-Hydroxylation in Monolignol Biosynthesis of Populus trichocarpa , MOLECULAR PLANT (2015)
- Improved Protocol for Alkaline Nitrobenzene Oxidation of Woody and Non-Woody Biomass , Journal of Wood Chemistry and Technology (2014)
- Systems Biology of Lignin Biosynthesis in Populus trichocarpa: Heteromeric 4-Coumaric Acid:Coenzyme A Ligase Protein Complex Formation, Regulation, and Numerical Modeling , The Plant Cell (2014)
Laccases were proposed for lignin biosynthesis and for phenolic production. However, currently there are no reports of laccase functions on the monolignol polymerization in trees. We have identified 20 xylem-expressed laccases among the 79 laccase gene models in the P. trichcarpa genome. To study which laccases are involved in wood formation, we produced transgenics overexpressing Ptr-miR397a, which is predicted to target laccases. Our preliminary qRT-PCR showed gene expression reductions of selected six laccase genes, indicating successful functioning of Ptr-miR397a in the transgenic plants. We propose to characterize the transgenic plants for transcriptome and sugar composition analysis, including lignin content, lignin S/G ratio, and phenolic content. Correlation of the transcriptome and sugar composition changes will enable us to identify the laccases responsible for wood formation.