Shi_PhD.Abstract.pdf

Abstract:
This project focuses on enhancing plant tissue culture and transformation efficiency through controlled environments. The study centers on soybean (Glycine max) as a crucial agronomic crop and aims to improve Agrobacterium-mediated transformation and shoot organogenesis in soybean using half-seeds. The research investigated the impact of light intensity, crossing wide range between 40 to 190 µmol · m¯² · s¯¹, during co-cultivation on the transformation efficiency and shoot organogenesis. Later, the research also explores the feasibility of photoautotrophic approach in Cannabis sativa micropropagation and the influence of air change rates on Cannabis shoots in traditional tissue culture and photoautotrophic culture. This project revealed that implementing photoautotrophic culture practices accelerated Cannabis micropropagation while maintaining similar outcomes to traditional sugar-based tissue culture. Additionally, increasing air change rate from 0.5 h ¯¹ to 14.5 h ¯¹ resulted in enhanced shoot growth and root development in photoautotrophic system. In summary, this project focuses on optimizing plant tissue culture and transformation processes in soybean by various light intensities and explores innovative approaches to enhance Cannabis micropropagation. The findings contribute to the advancement of plant biotechnology and have practical implications for crop improvement and efficient micropropagation techniques.