CALS Employee Portal Calendar
Seminar: Tim Kelliher – Disrupting Crop Breeding with Genome Editing and Haploid Induction
- This event has passed.
Event Navigation
Dr. Tim Kelliher
Principal Scientist, Reproduction Biology, Syngenta
Genome editing using CRISPR-Cas9 works efficiently in plant cells, but delivery of genome editing machinery into the vast majority of crop varieties is not possible using established methods. We co-opted the aberrant reproductive process of haploid induction (HI) to induce edits in nascent seeds of diverse monocot and dicot species. Our method, named “HI-Edit”, enables direct genomic modification of commercial crop varieties. HI-edit was tested in field and sweet corn using a native haploid inducer line, and extended to dicots using an engineered CENH3 HI system. We also recovered edited wheat embryos using Cas9 delivered by maize pollen. Our data indicate that a transient hybrid state precedes uniparental chromosome elimination in maize HI. Edited haploid plants lack both the haploid inducer parental DNA and the editing machinery. Therefore, edited plants could be used in trait testing and directly integrated into commercial variety development.
Participate via ZOOM: https://ncsu.zoom.us/j/760906613
Details
- Date
- November 18, 2019
- Time
-
3:00 pm - 4:00 pm
- Event Categories:
- Cals Research, Employee Portal Homepage, Horticultural Science, Horticulture, Seminar, Seminars
- Website
- https://ncsu.zoom.us/j/760906613
- 121 Kilgore Hall
-
2721 Founders Drive
Raleigh, NC 27606 United States - Website
- View Venue Website
Submit an event to the Employee Portal calendar
In compliance with the Americans with Disabilities Act, NC State will honor requests for reasonable accommodations made by individuals with disabilities. Direct accommodation requests to the event organizer listed on the event.