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Marc Cubeta

Professor and Associate Director, Center for Integrated Fungal Research

Ecology and population genetics of beneficial and plant pathogenic soil fungi

Partners Building III 225

919.513.1227

https://plantpath.cals.ncsu.edu/people/faculty/marc-cubeta/

Research: 

Fungi have evolved complex relationships with the environment, animals, insects, plants and microbes, that have ultimately contributed to their success in space and time. My research aims to gain better insight into the ecological factors and population processes that have contributed to the ability of fungi to cause disease and survive in the absence of a host; this requires a comprehensive understanding of species concepts, genome organization, evolution, and population genetics in the context of plant microbial interactions. This is critical for establishing a foundation of fundamental knowledge that can be translated for the practical management of disease causing fungi and the promotion of beneficial microorganisms that address societal grand challenges. Research in my laboratory has focused primarily on understanding the disease ecology, genome organization, soil microbiome, population biology, and systematics of the soil fungus Rhizoctonia solani, an economically important pathogen of a wide array of cultivated and native species of plants. Our research has revealed that R. solani is not a single species, but a species complex that represents an early diverging assemblage of fungi that may have given rise to the mushroom forming fungi. These results have provided a conceptual framework for delineating species, examining the genetic diversity and structure of field populations, determining the occurrence and transmission of fungal viruses, and identifying two previously undescribed Rhizoctonia 1) the disease-causing species of Rhizoctonia on agricultural plants and 2) beneficial species of Rhizoctonia on orchids. In addition to Rhizoctonia fungi, research from my lab has contributed to the development of methods for identifying other species of beneficial and disease-causing fungi of plants and more recently animals. My laboratory has also provided valuable bioinformatics, genomic, diagnostic, metabolomic, and microbiome resources for scientists interested in understanding the complex dynamics of different nuclear genomes in Rhizoctonia and other plant/soil associated beneficial and disease-causing fungi. This information has provided foundational knowledge that is key to conducting comparative studies that address fundamental research questions related to host plant adaptation in relation to nuclear genome heterogeneity.

Teaching: 

PP222 Kingdom of Fungi (fall and spring) – This course provides an overview of the diversity of fungi and their ecological, economic and historical impact on the environment and society. The fall course is a taught in a traditional classroom setting (face-to-face) and supplemented with the examination and discussion of fungal specimens. For the spring course, lectures, course content and discussions are presented and delivered in an online, distance education format.

PP575 Introduction to Mycology (fall, odd years) – This course provides a synthesis and survey of organisms in the Kingdom Fungi. Lectures focus on providing a comprehensive understanding of the biology, diversity, ecology, phylogeny, systematics, and taxonomy of fungi. The laboratory component of this course complement the lectures and involve characterizing, culturing and identifying fungi sampled from diverse ecological habitats.

Selected Peer-Reviewed Publications (last 10 years):

2021

Becker, L.E., Adhikari, U., Mays, T., Shishkoff, N., Crouch, J.A. and Cubeta, M.A. Evaluation of boxwood cultivars for resistance to boxwood blight. Plant Disease Management Reports 15: OT008.

LeBlanc, N., Cubeta, M.A., and Crouch, J. 2021. Population genomics trace clonal diversification and intercontinental migration of an emerging fungal disease of boxwood. Phytopathology 111(1):184-193, https://doi.org/10.1094/PHYTO-06-20-0219-FI. Invited submission for Special Population Focus Issus, Population Genomic and Phylogenomic Enabled Advances to Increase Insight Into Pathogen Biology and Epidemiology.

 Nowicki, M., Hadziabdic, D., Trigiano, R.N., Boggess, S.L., Kanetis, L., Wadl, P.A., Ojiambo, P., Cubeta, M.A., Spring, O., Thines, M., Runge, F., and Scheffler, B.E. 2021. ‘Jumping Jack’: Genomic microsatellites underscore the distinctiveness of closely related Pseudoperonospora cubensis and Pseudoperonospora humuli and provide new insights into their evolutionary past.
Frontiers in Microbiology https://doi.org/10.3389/fmicb.2021.686759

Shishkoff, N. Miller, M.E., and Cubeta, M.A. 2021. Rooting response of healthy boxwood cultivars to hot water treatment and thermal inactivation of Calonectria henricotiaeand  pseudonaviculata in diseased boxwood tissue. Journal of Environmental Horticulture 39(1):1-10, https://doi.org/10.24266/0738-2898-39.1.1

Stalpers, J., Redhead, S.A., Rossman, A., Crouch, J.A., Cubeta, M.A., Kirschner, R., Langer, G.J., Larsson, K-H., Mack, J., Marvanova, L., May, T.W., Norvell, L.L., Oberwinkler, F., Roberts, P., Rajchenberg, R., Seifert, K.A., and Thorn, R.G. 2021. Competing sexual-asexual generic names in the Agaricomycotina (Basidiomycota) with recommendations for use. IMA Fungus (In Press).

Toms, A., White, J.B., Mahmood, S., Finlay, R.D., Stenlid, J., Cubeta, M.A., and Carbone, I.C. 2021. Biodiversity of fungi associated with orchid soils in Sweden: a case study for increased phylogenetic resolution of taxonomic and trophic relationships. Evolutionary Applications (In review, manuscript number EA-20-0957).

Yow, A., Zhang, Y., Bansal, K, Maren, N., Eacker, S.M., Sullivan, S., Liachko, I., Cubeta, M.A., Rollins, J.A., and Ashrafi, H.. 2021. Genome sequence of Monilinia vaccini-corymbosi sheds light on mummy berry disease infection of blueberry and mating type. Genes Genomes Genetics 11(2),https://doi.org/10.1093/g3journal/jkaa052

2020

Becker L.E. and Cubeta M.A. 2020. Evaluation of calibrachoa cultivars for black root rot resistance, 2019. Plant Disease Management Reports 14: OT013.

Flemming, A., Phillips, M., Shea, E.K., Bolton, A., Lincoln, C., Green, K.E., Mast, A., and Cubeta, M.A. 2020. Using Digital Natural History Collections in K-12 STEM Education. Journal of Museum Education (Accepted, pending revision).

2019

Cullen, M., Jacob, M.E., Vanderschel, I.Q., Cotter, H.V.T., Cornish, V., Cubeta M.A., Carbone, I., and Gilger, B.C. 2019. Relationship of multi-locus DNA sequence analysis, anti-fungal agent susceptibility and equine fungal keratitis outcome. PLoS One, Mar 28;14(3):e0214214. doi:10.1371/journal.pone.0214214. eCollection 2019.

Green, K.E., Roller, C., and Cubeta, M.A. 2019. A plethora of fungi: developing mycological educational resources for biology and environmental science teachers and students. Science Activities ISSN: 0036-8121 (Print) 1940-1302 (Online) https://www.tandfonline.com/loi/vsca20.

Koehler, A.M., Larkin, M.T., Rogers, L.W., Carbone, I., Cubeta, M.A., and Shew, H.D. 2019. Identification and characterization of Septoria steviae as the causal agent of Septoria leaf spot disease of stevia in North Carolina. Mycologia, 111:3, 456-465, DOI: 10.1080/00275514.2019.1584503.

Salgado-Salazar, C., Shishkoff, N., LeBlanc, N., Ismaiel, A.A., Collins, M.D., Cubeta, M.A., and Crouch, J.A. 2019. Coccinonectria pachysandricola, causal agent of a new foliar blight disease of Sarcococca hookeriana. Plant Disease 103(4):1337-1346. https://doi.org/10.1094/PDIS-09-18-1676-RE.

2018

Lookabaugh, E.C., Kerns, J.P., Shew, B.B, and Cubeta, M.A. 2018. Fitness attributes of Pythium aphanidermatum with dual resistance to mefenoxam and fenamidone. Plant Disease 102(10). doi.org/10.1094/PDIS-01-18-0043-RE.

Miller, M.E., Shishkoff, N. and Cubeta, M.A. 2018. Thermal sensitivity of Calonectria henricotiae and Calonectria pseudonaviculata conidia and microsclerotia. Mycologia 110 (3): 546-558, doi:10.1080/002755514.2018.1465778.

2017

Burchhardt, K.M., Miller, M.E., Cline, W.O., and Cubeta, M.A. 2017. Fine-scale genetic structure and reproductive biology of the blueberry pathogen Monilinia vaccinii-corymbosi. Phytopathology 107:231-239. http://dx.doi.org/10.1094/PHYTO-02-16-0093-R.

Duckworth, O.W., Andrews, M.Y., Cubeta, M.A., Grunden, A.M., and Ojiambo, P. 2017. Revisiting graduate student training to address agricultural and environmental societal challenges. Agric. Environ. Lett. 2:170019, doi:10.2134/ael2017.06.0019.

2016

Cubeta, M.A., Mozley, S.E., and Porter, D. 2016. Laboratory Exercises with Zoosporic Fungi. In Plant Pathology Laboratory Exercises and Concepts. CRC Press, eds. Trigiano and Windham, pp. 99-110.

Gonzalez, D, Rodriguez-Carres, M., Boekhout, T., Stalper, J., Kurame, E.E., Nakatani, A.K., Vilgalys, R., and Cubeta, M.A. 2016. Phylogenetic relationships of Rhizoctonia fungi within the Cantharellales. Fungal Biology 120:603-619.

Pettersson, M., Frampton, J., Rönnberg, J., Shew, H.D., Benson, D.M., Kohlway, W.H., Escanferla, M.E., and Cubeta, M.A. 2016. Increased diversity of Phytophthora Species in Fraser fir Christmas Tree Plantations in the Southern Appalachians. Scandinavian Journal of Forestry http://www.tandfonline.com/doi/full/10.1080/02827581.2016.1265144.

2015

Burchhardt, K.M. and Cubeta, M.A. 2015. Population dynamics of the blueberry pathogen Monilinia vaccinii-corymbosi in the United States. Phytopathology 105:1-9 (selected as Editor’s pick for June).

Chamoun, R., Samsatly, J., Pakala, S., Cubeta, M.A., and Jabaji, S. 2015. Suppression subtractive hybridization and comparative expression of a pore-forming toxin and glycosyl hydrolase genes in Rhizoctonia solani during potato sprout infection. Molecular Genetics and Genomics: DOI: 10.1007/s00438-014-0962-x.

Gkarmiri, K., Finlay, R.D., Alström, S., Thomas, E., Cubeta, M.A., and Högberg, N. 2015. Transcriptomic analyses of changes in gene expression in the fungal pathogen Rhizoctonia solani AG-3 in response to the bacterial antagonists Serratia proteamaculans and Serratia plymuthica. BMC Genomics 16:630 DOI 10.1186/s12864-015-1758-z.

Jr-Hau Jiang, J-H., Lee, Y-I., Cubeta, M.A., and Chen, L-C. 2015. Characterization and colonization of endomycorrhizal Rhizoctonia fungi in the medicinal herb Anoectochilus formosanus (Orchidaceae). Mycorrhizae DOI 10.1007/s00572-014-0616-1.

Mothapo, N.V. Chen, H., Cubeta, M.A., Grossman, J.M., Fuller, F, and Shi, W. 2015. Phylogenetic, taxonomic and functional diversity of fungal denitrifiers and associated N2O production efficacy. Soil Biology & Biochemistry 83:160-175.

2014

Bazzle, L., Cubeta, M.A., Marks, S.L., and Dorman, D.C. 2014. Feasibility of flotation concentration of fungal spores as a method to identify toxigenic mushrooms. Vet Med Res Reports. (In Press).

Burchhardt, K.M. and Cubeta, M.A. 2014. Population dynamics of the blueberry pathogen Monilinia vaccinii-corymbosi in the United States. Phytopathology 104: (Accepted, manuscript number PHYTO-03-14-0074-R).

Chamoun, R., Samsatly, J., Pakala, S., Cubeta, M.A., and Suha Jabaji, S. 2014. Suppression subtractive hybridization and comparative expression of a pore-forming toxin and glycosyl hydrolase genes in Rhizoctonia solani during potato sprout infection. Molecular Genetics and Genomics: (Accepted, manuscript number MGAG-D-14-00189).

Jr-Hau Jiang, J-H., Lee, Y-I., Cubeta, M.A., and Chen, L-C. 2014. Characterization and colonization of endomycorrhizal Rhizoctonia fungi in the medicinal herb Anoectochilus formosanus(Orchidaceae). Mycorrhizae. (Accepted, manuscript number MCOR-D-14-00082).

Losada, L., Pakala, S.B., Fedorova, N.D., Joardar, V., Shabalina. S., Hostetler, J., Pakala, S., Zafar, N., Thomas, E.T., Rodriguez-Carres, M., Dean R.A., Vilgalys, R.J., Nierman, W.C., and Cubeta, M.A.2014. Mobile intron-like elements and novel mitochondrial genome expansion in the soil fungus and potato pathogen Rhizoctonia solani. FEMS Letters 350:1-11 (DOI: 10.1111/1574-6968.12387).

 2013

Bartz, F.E., Danehower, D.A., Glassbrook, N., and Cubeta, M.A. 2013. Modulation of the phenylacetic acid metabolic complex by quinic acid alters the disease-causing activity of Rhizoctonia solani on tomato. Phytochemistry 75: http://dx.doi.org/10.1016/j.phytochem.2012.09.018.

Ferrucho, R.L., Garcia-Dominguez, C., Ramirez-Escobar, U.M., McDonald, B. A., Cubeta, M.A., and P.C. Ceresini, P.C. 2013. The population genetic structure of Rhizoctonia solani AG-3PT from potatoes in the Colombian Andes. Phytopathology 103:862-869.

McCormick, M.A., Grand, L.F., Post, J.B., and Cubeta, M.A. 2013. Phylogenetic relatedness and phenotypic characterization of Fomes fasciatus and Fomes fomentarius sampled from the United States. Mycologia 105(6):1524-1535.

McCormick, M.A., Cubeta, M.A., and Grand, L.F. 2013. Geography and hosts of the wood decay fungi Fomes fasciatus and Fomes fomentarius in the United States. North American Fungi 8(2):1-53. doi:10.2509/naf2013.008.002.

Mothapo, N.V, Chen, H., Cubeta, M.A., and Shi, W. 2013. Nitrous oxide producing activity of diverse fungi from distinct agroecosystems. Soil Biology & Biochemistry 66:94-101.

Skamnaki, V., Peumans, W., Kantsadi, A., Cubeta, M.A., Plas, K., Pakala, S., Zographos, S., Smagghe, G., Nierman, W.C., Van Damme, E., and Leonidas, D. 2013. Structural analysis of the Rhizoctonia solani agglutinin reveals an unusual dimeric assembly. FEBS Journal 280:1750-1763.

 2012

Bartz, F.E., Danehower, D.A., Glassbrook, N., and Cubeta, M.A. 2012. Elucidating the role of phenylacetic acid and hydroxy- and methoxy- phenylacetic acid derivatives in the pathogenic activity of Rhizoctonia solani AG-3. Mycologia 104:793-803.

Burchhardt, K. and Cubeta, M.A. 2012. Microsatellite marker development for the plant pathogenic fungus Monilinia vaccinii-corymbosi. Molecular Ecology Resources 12:72-74.

Mozley, S.E., Porter, D, and Cubeta, M.A. 2012. Concepts and Laboratory Exercises with Zoosporic Fungi. In Plant Pathology Laboratory Exercises and Concepts. CRC Press, eds. Trigiano and Windham, pp. 91-98.

Samuels, G., Ismaiel, A., McMahon, P., Guest, D., Rosmana, A., Junaid, M., Rodriguez-Carres, M., and Cubeta, M.A. 2012. Vascular streak dieback of cacao in Southeast Asia and Melanesia: in planta detection of the pathogen and a new taxonomy. Fungal Biology 116(1):11-23.

Thomas, E., Pakala, S., Fedorova, N. D., Nierman, W. C., and Cubeta, M. A. 2012. Triallelic SNP-mediated genotyping of regenerated protoplasts of the heterokaryotic fungus Rhizoctonia solani. Journal of Biotechnology 158:144-150. 

Education

BS, Plant and Soil Science, University of Delaware (1980)
MS, Plant Pathology/Soil Microbiology, University of Illinois (1983)
Ph.D, Plant Pathology, North Carolina State University (1991)