Manuel Kleiner
Associate Professor
Microbiomes and Complex Microbial Communities Cluster
Thomas Hall 4510B
Bio
Microbial communities are ubiquitous in all environments on Earth that support life, and they play crucial roles in global biogeochemical cycles, plant and animal health, and biotechnological processes. However, most microbial species from a given habitat cannot be cultured and thus cannot be experimentally characterized in the laboratory. Therefore, to study environmental microbes we rely on so-called cultivation-independent methods that allow us to study microorganisms directly in their environment.
We study the metabolism, physiology, and evolutionary ecology of microbial symbioses and uncultured microorganisms. To this end we develop and use cultivation-independent approaches such as metagenomics, metaproteomics, and metabolomics, as well as more targeted approaches such as enzyme assays, single-cell imaging methods, and stable isotope-based experiments. We combine the study of uncultured microorganisms with genetic, molecular, and biochemical studies on cultivable microorganisms to gain an in-depth understanding of specific metabolic pathways and physiological strategies.
The current projects focus on:
- Factors governing energy efficiency of metabolism in free-living and symbiotic bacteria, looking specifically at a novel CO2 fixation pathway
- The role of horizontal gene transfer in the metabolic evolution of bacterial symbionts
- Development of cutting-edge methods for microbial community analyses focusing on metagenomics and high-end mass spectrometry based metaproteomics
For a more detailed description of our projects, visit the Kleiner Lab website.
View Publications on Google Scholar
Education
Ph.D. Marine Microbiology Max Planck Institute for Marine Microbiology, Germany 2012
Diploma Biology University of Greifswald, Germany 2008
Area(s) of Expertise
Microbial physiology and metabolism, bacteria-animal symbiosis , metagenomics and metaproteomics, environmental microbiology, marine microbiology, and renewable resources
Publications
- IPD3, a master regulator of arbuscular mycorrhizal symbiosis, affects genes for immunity and metabolism of non-host Arabidopsis when restored long after its evolutionary loss , PLANT MOLECULAR BIOLOGY (2024)
- Metabolically-versatileCa.Thiodiazotropha symbionts of the deep-sea lucinid clamLucinoma kazanihave the genetic potential to fix nitrogen , (2024)
- Metaproteomics and DNA metabarcoding as tools to assess dietary intake in humans , (2024)
- De novo phytosterol synthesis in animals , SCIENCE (2023)
- Effective seed sterilization methods require optimization across maize genotypes , (2023)
- Environmental legacy effects impact maize growth and microbiome assembly under drought stress , (2023)
- Evaluation of ready-to-use freezer stocks of a synthetic microbial community for maize root colonization , MICROBIOLOGY SPECTRUM (2023)
- Evaluation of ready-to-use freezer stocks of a synthetic microbial community for maize root colonization , (2023)
- Large Quantities of Bacterial DNA and Protein in Common Dietary Protein Source Used in Microbiome Studies , (2023)
- Mechanism of high energy efficiency of carbon fixation by sulfur-oxidizing symbionts revealed by single-cell analyses and metabolic modeling , (2023)