Xiaojing Liu

Bio

Research Interests

I am a bioanalytical chemist investigating the fundamental principles of metabolic regulation within intestinal tissues. Operating on the philosophy that metabolic rewiring is a primary driver of disease dynamics—not just a passenger—my research program spans cellular biochemistry to whole-organism physiology to decode complex pathologies. Core research areas include:

• Advanced Multi-Omics Workflows: Pioneering integrative liquid chromatography–high-resolution mass spectrometry (LC-HRMS) pipelines across metabolomics, lipidomics, proteomics, and phosphoproteomics in both cell-based and whole-organism models.
• Intestinal Metabolic Regulation: Investigating how localized metabolic shifts and tissue-specific mitochondrial function govern systemic health and disease.
• Mechanistic Analysis of Drug Action: Bridging cellular biochemistry with organ-specific responses, with a particular focus on how mitochondrial fuel plasticity dictates drug sensitivity (e.g., metformin).
• Stable Isotope Flux Analysis: Developing sophisticated in vitro and in vivo tracing methodologies (using 13C-glucose, 13C-glutamine, and 18O tracing) to quantitatively map pathway contributions to specific biological targets.

Area(s) of Expertise

Multi-Omics for Drug Mechanisms

Publications

• 62. Metabolomic Analyses of Uterine Luminal Fluid Reveals Regulatory Landscapes During the Peri-implantation Period of Pregnancy in Pigs , Journal of Animal Science (2026)
• Androgen excess uncouples circulating and hepatic lipid homeostasis in females on a high-calorie diet , Journal of Lipid Research (2026)
• Author Correction: Regulation of urea cycle by reversible high-stoichiometry lysine succinylation , Nature Metabolism (2026)
• Solvatochromic BODIPYs with Polarity-Independent Absorption for Functional Super-resolution Imaging of Live Cell Membranes , Chemical & Biomedical Imaging (2025)
• The Heme Oxygenase-Like Diiron Enzyme HrmI Reveals Altered Regulatory Mechanisms for Dioxygen Activation and Substrate N-Oxygenation , Journal of the American Chemical Society (2025)
• Data-dependent and -independent acquisition lipidomics analysis reveals the tissue-dependent effect of metformin on lipid metabolism , Metabolomics (2024)
• Fructose regulates the pentose phosphate pathway and induces an inflammatory and resolution phenotype in Kupffer cells , Scientific Reports (2024)
• Metabolic regulation of the glioblastoma stem cell epitranscriptome by malate dehydrogenase 2 , Cell Metabolism (2024)
• Myeloid cell MHC I expression drives CD8+ T cell activation in nonalcoholic steatohepatitis , Frontiers in Immunology (2024)
• Quantitation of metabolic activity from isotope tracing data using automated methodology , Nature Metabolism (2024)