Bob Rose
Education
B.A. Physics Yale College 1982
M.A. History of Science University of California, Berkeley 1989
Ph.D. Biophysics University of California, San Francisco 1996
Post-doctoral Molecular and Cell Biology University of California, Berkeley 2002
Area(s) of Expertise
Our lab studies the structure and function of carbon fixing enzymes. Our goal is to engineer these enzymes to capture atmospheric CO2, the most abundant greenhouse gas. Currently we are studying the multi-domain biotin-dependent carboxylase complexes. These enzymes include pyruvate carboxylase and acetyl-CoA carboxylase which play essential roles in central metabolism and fat biosynthesis. We are studying the assembly of a unique thermophilic member of this family, Oxoglutarate Carboxylase (OGC), that carboxylase 2-oxogluatrate. Structural comparisons and the temperature-dependence of the enzyme kinetics provide insights into the rate-determining steps of the enzyme. Our goal is to identify mutations in the enzyme that optimize its activity at mesophilic temperatures. This will allow OGC to be added to a synthetic carbon fixation cycle designed to enhance the growth rate of plants utilized as feedstock for biofuels.
Recent publication:
Buhrman G, Enríquez P, Dillard L, Baer H, Truong V, Grunden AM, Rose RB. Structure, Function, and Thermal Adaptation of the Biotin Carboxylase Domain Dimer from Hydrogenobacter thermophilus 2-Oxoglutarate Carboxylase. Biochemistry. 2021 Feb 2;60(4):324-345
Publications
- Characterization of an isobutylene epoxide hydrolase (IbcK) from the isobutylene-catabolizing bacterium Mycolicibacterium sp. ELW1 , Applied and Environmental Microbiology (2025)
- Structural and kinetic characterization of an acetoacetyl-Coenzyme A: acetate Coenzyme A transferase from the extreme thermophile Thermosipho melanesiensis , Biochemical Journal (2025)
- Binding specificity and function of the SWI/SNF subunit SMARCA4 bromodomain interaction with acetylated histone H3K14 , Journal of Biological Chemistry (2021)
- Structure, Function, and Thermal Adaptation of the Biotin Carboxylase Domain Dimer from Hydrogenobacter thermophilus 2-Oxoglutarate Carboxylase , Biochemistry (2021)
- 5-Hydroxymethylcytosine in E-box motifs ACAT|GTG and ACAC|GTG increases DNA-binding of the B-HLH transcription factor TCF4 , Integrative Biology (2016)
- A High-Resolution Crystal Structure of a Psychrohalophilic α–Carbonic Anhydrase from Photobacterium profundum Reveals a Unique Dimer Interface , PLoS ONE (2016)
- Modeling the Growth of Archaeon Halobacterium halobium Affected by Temperature and Light , Applied Biochemistry and Biotechnology (2016)
- Evaluation of a DLA-79 allele associated with multiple immune-mediated diseases in dogs , Immunogenetics (2015)
- Carboxylation of cytosine (5caC) in the CG dinucleotide in the E-box motif (CGCAG|GTG) increases binding of the Tcf3|Ascl1 helix-loop-helix heterodimer 10-fold , Biochemical and Biophysical Research Communications (2014)
- Interactions with the Bifunctional Interface of the Transcriptional Coactivator DCoH1 Are Kinetically Regulated , Journal of Biological Chemistry (2014)