Trees are the most abundant natural resource living on Earth\u2019s land masses, and North Carolina State University scientists and engineers are making headway in finding ways to use them as sustainable, environmentally benign alternatives to producing industrial chemicals from petroleum.<\/p>\n
Lignin, a polymer that makes trees rigid and resistant to degradation, has proven problematic. Now those NC State researchers know why: They\u2019ve identified the specific molecular property of lignin \u2014 its methoxy content \u2014 that determines just how hard, or easy, it would be to use microbial fermentation to turn trees and other plants into industrial chemicals.<\/p>\n
The findings put us a step closer to making industrial chemicals from trees as an economically and environmentally sustainable alternative to chemicals derived from petroleum, said Robert Kelly, the corresponding author of a paper in the journal Science Advances<\/em> detailing the discovery<\/a>.<\/p>\n Kelly\u2019s group previously proved that certain extreme thermophilic bacteria, which thrive in places such as Yellowstone National Park hot springs, can degrade the cellulose in trees \u2014 but \u201cnot to a great extent,\u201d he said. \u201cIn other words, not at the level that would make economic and environmental sense for producing industrial chemicals.\u201d<\/p>\n As Kelly explained, \u201cIt turns out that there\u2019s more than just low lignin at play.\u201d<\/p>\n To get around the high lignin problem with trees, Kelly, the director of NC State\u2019s Biotechnology Program and Alcoa Professor in the Department of Chemical and Biomolecular Engineering, has been working for over 10 years with Associate Professor Jack Wang, the head of the Forest Biotechnology Program in NC State\u2019s College of Natural Resources. Wang is also a faculty member with the N.C. Plant Sciences Initiative.<\/p>\n As reported in the journal Science in 2023<\/a>, Wang and his colleagues used CRISPR genome editing technology to create poplar trees with modified lignin content and composition. They have focused on poplar trees because they are fast growing, require minimal use of pesticides and grow on marginal lands that are hard to grow food crops on.<\/p>\n Kelly\u2019s group found that some, but not all, of these CRISPR-edited trees worked well for microbial degradation and fermentation. As his former Ph.D. student Ryan Bing explained, it turns out that these bacteria have different appetites for different types of plants.<\/p>\n \u201cWe can harness the ability of certain thermophilic bacteria from hot springs in places like Yellowstone National Park to eat the plant matter and convert it to products of interest. However, these bacteria have varying appetites for different types of plants,\u201d said Bing, who now works as senior metabolic engineer for Capra Biosciences in Sterling, Virginia.<\/p>\n \u201cThe question was why? What makes one plant better than the next?\u201d he explained. \u201cWe found an answer to this by looking at how these bacteria eat plant matter of various compositions.\u201d <\/p>\n In a follow-up study, Kelly and Bing tested how well a genetically engineered bacterium originally isolated from hot springs in Kamchutka, Russia, Anaerocellum bescii<\/em>, broke down Wang\u2019s engineered poplar trees with markedly different lignin contents and composition.<\/p>\n The researchers found that the lower the tree\u2019s lignin methoxy content was, the more degradable it was.<\/p>\n \u201cThis cleared up the mystery of why lower lignin alone is not the key \u2014 the devil was in the details,\u201d Kelly said. \u201cLow methoxy content likely makes the cellulose more available to the bacteria.\u201d<\/p>\n Wang had created the low-lignin poplars to be better for papermaking and other fiber products, but the recent research suggests that engineered poplars that have not just low lignin but also low methoxy content are best for making chemicals through microbial fermentation.<\/p>\n Wang\u2019s engineered poplars grow well in the greenhouse, but results aren\u2019t in yet from field testing. Kelly\u2019s group has previously shown that low lignin poplar trees can be converted to industrial chemicals, such as acetone and hydrogen gas, with favorable economic outcomes as well as low environmental impact.<\/p>\n If these trees hold up in the field and \u201cif we keep working on our end,\u201d Kelly said, \u201cwe will have microbes that make large amounts of chemicals from poplar trees, now that we know the marker to look for \u2014 the methoxy content.\u201d<\/p>\n This gives researchers, like Wang, a specific target for producing poplar lines best suited for chemical production. Wang and colleagues have recently initiated field trials of advanced lignin modified poplar trees to address this question.<\/p>\n Right now, making chemicals from trees is doable by traditional means \u2014 chopping the wood into smaller pieces and then using chemicals and enzymes to pretreat it for further processing.<\/p>\n Using engineered microbes to break down lignin offers advantages, including lower energy requirements and lower environmental impact, Kelly said.<\/p>\n Enzymes can be used to break down cellulose into simple sugars, but they continually need to be added to the process. Certain microorganisms, on the other hand, continually produce the key enzymes that make the microbial process more economical, he said.<\/p>\n \u201cThey also can do a much better job than enzymes and chemicals,\u201d Kelly added. \u201cThey not only break down the cellulose but also ferment it to products, such as ethanol \u2013 all in one step.<\/p>\n \u201cThe high temperatures that these bacteria grow at also avoid the need to work under sterile conditions, as you would need to do with less thermophilic microorganisms to avoid contamination,\u201d he added. \u201cThis means that the process for turning trees into chemicals can operate like a conventional industrial process, making it more likely to be adopted.\u201d<\/p>\n Daniel Sulis, another author on the Science Advances<\/em> paper and a postdoctoral researcher in Wang\u2019s lab, said that environmental disasters fueled by climate change highlight the urgent need to conduct research that finds ways to reduce dependence on fossil fuels.<\/p>\n \u201cOne promising solution lies in harnessing trees to meet society’s needs for chemicals, fuels and other bio-based products while safeguarding both the planet and human well-being,\u201d Sulis added.<\/p>\n \u201cThese findings not only move the field forward but also lay the groundwork for further innovations in using trees for sustainable bio-based applications.\u201d<\/p>\n -shore-<\/p>\n Note to editors<\/strong>: The paper’s abstract follows.<\/p>\n \u201cBeyond low lignin: Identifying the primary barrier to plant biomass conversion by fermentative bacteria\u201d<\/strong> Published: Oct. 18, 2024 in Science Advances<\/em><\/p>\n DOI: 10.1126\/sciadv.adq4941<\/p>\n Abstract<\/strong>: Renewable alternatives for nonelectrifiable fossil-derived chemicals are needed and plant matter, the most abundant biomass on earth, provide an ideal feedstock. However, the heterogeneous polymeric composition of lignocellulose makes conversion difficult. Lignin presents a formidable barrier to fermentation of nonpretreated biomass. Extensive chemical and enzymatic treatments can liberate fermentable carbohydrates from plant biomass, but microbial routes offer many advantages, including concomitant conversion to industrial chemicals. Here, testing of lignin content of non-pretreated biomass using the cellulolytic thermophilic bacterium, Anaerocellum bescii<\/em>, revealed that the primary microbial degradation barrier relates to methoxy substitutions in lignin. This contrasts with optimal lignin composition for chemical pretreatment that favors high S\/G ratio and low H lignin. Genetically modified poplar trees with diverse lignin compositions confirm these findings. In addition, poplar trees with low methoxy content achieve industrially relevant levels of microbial solubilization without any pretreatments and with no impact on tree fitness in greenhouse.<\/p><\/p>\n This post was originally published<\/a> in NC State News.<\/em><\/p>","protected":false,"raw":" Trees are the most abundant natural resource living on Earth\u2019s land masses, and North Carolina State University scientists and engineers are making headway in finding ways to use them as sustainable, environmentally benign alternatives to producing industrial chemicals from petroleum.<\/p>\n\n Lignin, a polymer that makes trees rigid and resistant to degradation, has proven problematic. Now those NC State researchers know why: They\u2019ve identified the specific molecular property of lignin \u2014 its methoxy content \u2014 that determines just how hard, or easy, it would be to use microbial fermentation to turn trees and other plants into industrial chemicals.<\/p>\n\n The findings put us a step closer to making industrial chemicals from trees as an economically and environmentally sustainable alternative to chemicals derived from petroleum, said Robert Kelly, the corresponding author of a paper in the journal Science Advances<\/em> detailing the discovery<\/a>.<\/p>\n\n Kelly\u2019s group previously proved that certain extreme thermophilic bacteria, which thrive in places such as Yellowstone National Park hot springs, can degrade the cellulose in trees \u2014 but \u201cnot to a great extent,\u201d he said. \u201cIn other words, not at the level that would make economic and environmental sense for producing industrial chemicals.\u201d<\/p>\n\n As Kelly explained, \u201cIt turns out that there\u2019s more than just low lignin at play.\u201d<\/p>\n\n To get around the high lignin problem with trees, Kelly, the director of NC State\u2019s Biotechnology Program and Alcoa Professor in the Department of Chemical and Biomolecular Engineering, has been working for over 10 years with Associate Professor Jack Wang, the head of the Forest Biotechnology Program in NC State\u2019s College of Natural Resources. Wang is also a faculty member with the N.C. Plant Sciences Initiative.<\/p>\n\n
Authors: Ryan G. Bing, Daniel B. Sulis, Morgan J. Carey, Mohamad J. H. Manesh, Kathryne C. Ford, Christopher T. Straub , Tunyaboon Laemthong, Benjamin H. Alexander and Daniel J. Willard, NC State University; Xiao Jiang, University of Georgia; and Chenmin Yang, Jack P. Wang, Michael W.W. Adams, and Robert M. Kelly, NC State University<\/p>\n