{"id":10737,"date":"2026-09-01T15:12:38","date_gmt":"2026-09-01T19:12:38","guid":{"rendered":"https:\/\/cals.ncsu.edu\/international-programs\/?p=10737"},"modified":"2026-09-01T15:12:38","modified_gmt":"2026-09-01T19:12:38","slug":"plant-path-japan","status":"publish","type":"post","link":"https:\/\/cals.ncsu.edu\/international-programs\/news\/plant-path-japan\/","title":{"rendered":"Plant Pathology Research in Japan"},"content":{"rendered":"<h3>Summer 2026<\/h3>\n<p><strong>Caryn\u00a0<\/strong><strong>Biesecker<\/strong><\/p>\n<p>During this summer, I was provided the opportunity to gain undergraduate research experience in Dr. Daigo Takemoto&#8217;s Plant Pathology Laboratory at Nagoya University in Japan, under the mentorship of Maurizio Camagna. My research focused on understanding how solanaceous plants, like potatoes, tomatoes, and petunias, produce phytoalexins. Phytoalexins are antimicrobial compounds that help protect plants from pathogenic fungi. Specifically, I worked to identify a gene involved in the production of lubimin, a defense compound in the potato PVS pathway.<\/p>\n<div>My primary role was to clone candidate petunia genes\u00a0into expression vectors for future analysis of their role in lubimin production. In the laboratory, I designed primers and performed PCR, agarose gel electrophoresis, bacterial transformations, and plasmid preparations. I also learned more about and gained exposure to GC-MS, which can be used to analyze the compounds produced by the expressed genes. As I became more familiar with the laboratory protocols, I was able to perform many of these procedures independently.<\/div>\n<div><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-10739\" src=\"https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-768x1024.jpg\" alt=\"Caryn pipetting in the lab\" width=\"768\" height=\"1024\" srcset=\"https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-768x1024.jpg 768w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-225x300.jpg 225w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-1152x1536.jpg 1152w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-1536x2048.jpg 1536w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-1500x2000.jpg 1500w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-1200x1600.jpg 1200w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-1024x1365.jpg 1024w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-950x1267.jpg 950w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-600x800.jpg 600w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-460x613.jpg 460w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-300x400.jpg 300w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-230x307.jpg 230w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-scaled.jpg 1920w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/div>\n<div><\/div>\n<div>Outside of the laboratory, I was able to travel to many places in Japan and experience different cities, regions, and cultural traditions.\u00a0 Exploring Japan independently allowed me to become more comfortable navigating unfamiliar environments and communicating across language and cultural differences. I was able to experience local food, historical sites, and everyday life beyond the laboratory setting, which gave me a greater appreciation for Japanese culture and perspectives.<\/div>\n<div><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-10740\" src=\"https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-768x1024.jpg\" alt=\"Caryn by the Nagoya Tower\" width=\"768\" height=\"1024\" srcset=\"https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-768x1024.jpg 768w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-225x300.jpg 225w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-1152x1536.jpg 1152w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-1536x2048.jpg 1536w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-1500x2000.jpg 1500w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-1200x1600.jpg 1200w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-1024x1365.jpg 1024w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-950x1267.jpg 950w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-600x800.jpg 600w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-460x613.jpg 460w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-300x400.jpg 300w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-230x307.jpg 230w, https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-scaled.jpg 1920w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/div>\n","protected":false,"raw":"<h3>Summer 2026<\/h3>\r\n<strong>Caryn\u00a0<\/strong><strong>Biesecker<\/strong>\r\n\r\nDuring this summer, I was provided the opportunity to gain undergraduate research experience in Dr. Daigo Takemoto's Plant Pathology Laboratory at Nagoya University in Japan, under the mentorship of Maurizio Camagna. My research focused on understanding how solanaceous plants, like potatoes, tomatoes, and petunias, produce phytoalexins. Phytoalexins are antimicrobial compounds that help protect plants from pathogenic fungi. Specifically, I worked to identify a gene involved in the production of lubimin, a defense compound in the potato PVS pathway.\r\n<div>My primary role was to clone candidate petunia genes\u00a0into expression vectors for future analysis of their role in lubimin production. In the laboratory, I designed primers and performed PCR, agarose gel electrophoresis, bacterial transformations, and plasmid preparations. I also learned more about and gained exposure to GC-MS, which can be used to analyze the compounds produced by the expressed genes. As I became more familiar with the laboratory protocols, I was able to perform many of these procedures independently.<\/div>\r\n<div><img class=\"aligncenter size-large wp-image-10739\" src=\"https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/Japan3-768x1024.jpg\" alt=\"Caryn pipetting in the lab\" width=\"768\" height=\"1024\" \/><\/div>\r\n<div><\/div>\r\n<div>Outside of the laboratory, I was able to travel to many places in Japan and experience different cities, regions, and cultural traditions.\u00a0 Exploring Japan independently allowed me to become more comfortable navigating unfamiliar environments and communicating across language and cultural differences. I was able to experience local food, historical sites, and everyday life beyond the laboratory setting, which gave me a greater appreciation for Japanese culture and perspectives.<\/div>\r\n<div><img class=\"aligncenter size-large wp-image-10740\" src=\"https:\/\/cals.ncsu.edu\/international-programs\/wp-content\/uploads\/sites\/23\/2026\/09\/NCJapanCenter-768x1024.jpg\" alt=\"Caryn by the Nagoya Tower\" width=\"768\" height=\"1024\" \/><\/div>"},"excerpt":{"rendered":"<p>Summer 2026 Caryn\u00a0Biesecker During this summer, I was provided the opportunity to gain undergraduate research experience in Dr. Daigo Takemoto&#8217;s Plant Pathology Laboratory at Nagoya University in Japan, under the mentorship of Maurizio Camagna. My research focused on understanding how [&hellip;]<\/p>\n","protected":false},"author":914,"featured_media":10738,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"source":"","footnotes":"","_links_to":"","_links_to_target":""},"categories":[110],"tags":[],"class_list":["post-10737","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-research-pack-abroad"],"acf":[],"flickr_id":"","youtube_id":"","_links":{"self":[{"href":"https:\/\/cals.ncsu.edu\/international-programs\/wp-json\/wp\/v2\/posts\/10737","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cals.ncsu.edu\/international-programs\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cals.ncsu.edu\/international-programs\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cals.ncsu.edu\/international-programs\/wp-json\/wp\/v2\/users\/914"}],"replies":[{"embeddable":true,"href":"https:\/\/cals.ncsu.edu\/international-programs\/wp-json\/wp\/v2\/comments?post=10737"}],"version-history":[{"count":1,"href":"https:\/\/cals.ncsu.edu\/international-programs\/wp-json\/wp\/v2\/posts\/10737\/revisions"}],"predecessor-version":[{"id":10741,"href":"https:\/\/cals.ncsu.edu\/international-programs\/wp-json\/wp\/v2\/posts\/10737\/revisions\/10741"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cals.ncsu.edu\/international-programs\/wp-json\/wp\/v2\/media\/10738"}],"wp:attachment":[{"href":"https:\/\/cals.ncsu.edu\/international-programs\/wp-json\/wp\/v2\/media?parent=10737"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cals.ncsu.edu\/international-programs\/wp-json\/wp\/v2\/categories?post=10737"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cals.ncsu.edu\/international-programs\/wp-json\/wp\/v2\/tags?post=10737"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}