Date: Monday, March 21, 2022
Time: 3:00 pm
Speaker: Xiaonan Shi, PhD Introduction Seminar
Title: Optimization of Controlled Environment on Plant Tissue Culture in Shoot Organogenesis of Soybean (Glycine max) and Micropropagation of Hemp (Cannabis sativa)
Host: Dr. Ricardo Hernandez121 Kilgore / Hybrid
Join Zoom Meeting: https://ncsu.zoom.us/j/91288906768?pwd=Zy9ESnAycTVSUVdydnJWTzRyUFE3dz09
Meeting ID: 912 8890 6768
Passcode: 605122

Abstract:
Plant tissue culture plays a crucial role in micropropagation and plant biotechnology for the purpose of virus elimination, shoot multiplication, and regeneration of genetically transformed plants. Incubating conditions of plant tissue culture are set at certain temperature, light intensity, and photoperiod. The highly controlled environmental settings in vitro bear resemblance as well as difference to controlled environment for plant growing outside vessels. The unique, yet unfavorable elements to photosynthesis in controlled environments of plant tissue culture are the limited air exchange, due to aseptic requirement, and the relatively low light intensity. Another challenge of plant tissue culture is contamination that is often initiated on sugar-based medium. Plants in vitro possess photosynthetic capacity to a certain degree. It has been established that increasing light intensity increases plant growth ex vitro. Under limited air exchange conditions, a question remains whether various light intensity could affect plants in vitro. Photoautotrophic culture is defined as micropropagation without sugar in the medium. It could reduce contamination and alleviate acclimation of the culture after the rooting process. Shoot organogenesis is the critical step of Agrobacterium-mediated transformation in soybean (Glycine max), where shoot meristem generates potentially transformed shoots. Micropropagation of emerging specialty crop hemp (Cannabis sativa) is the other interest of this project, where nodal segments are multiplied for large quantities of shoots. The overall objective of this project is to improve plant tissue culture conditions by optimizing light intensities of white LEDs in soybean shoot organogenesis using half-seed explant and implementing photoautotrophic approach in hemp micropropagation.