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Seminar: Lena Wilson: Bulked-Segregant RNA-Seq of Three Firm-Fruited Selections vs. Three Soft-Fruited Selections of Southern Highbush Blueberry (Vaccinium corymbosum hybrid)

March 28 | 1:00 pm - 2:00 pm

Bulked-Segregant RNA-Seq of Three Firm-Fruited Selections vs. Three Soft-Fruited Selections of Southern Highbush Blueberry (Vaccinium corymbosum hybrid)
Lena Wilson, MS Seminar
Under the direction of Dr. Hamid Ashrafi
Monday, March 28, 2022 at 1:00pm121 Kilgore / Hybrid
Join Zoom Meeting: https://ncsu.zoom.us/j/93209309311?pwd=aVdSSnp6c0ora2UxTG82aStHUU01QT09
Meeting ID: 932 0930 9311
Passcode: 236250

 

Abstract: Blueberries (Vaccinium corymbosum L.) benefit from increased fruit firmness because of consumer preference and machine harvestability. However, the genetic component of fruit texture and skin thickness and their relationship to firmness have yet to be deciphered. This study used bulked segregant RNA-seq (BSR-seq) for differential gene expression analysis. Previously an F1 population of a cross between firm-fruited southern highbush cv. ‘Reveille’ and soft-fruited cv. ‘Arlen’ was developed in our laboratory. The total RNA of the parents, the two softest, and the two firmest progenies were extracted at the breaker and fully ripe stages. Next-generation sequencing cDNA libraries were constructed and subjected to Illumina short-read sequencing. Subsequently, the short reads were mapped to a blueberry genome, and differentially expressed genes (DEGs) were identified in immature and mature fruit that coded for potentially biologically significant proteins such as expansins, polygalacturonase, polygalacturonase-inhibiting protein, and mannosidase. Additionally, DEGs corresponding to cysteine proteases and S-adenyl methyltransferases (SAM-MTases) that were previously reported as candidate genes for blueberry firmness were identified in this study. Our results indicated that BSR-seq is a promising method for identifying candidate genes controlling complex traits in blueberry.

Details

Date:
March 28
Time:
1:00 pm - 2:00 pm
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