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Paul Labadie

Agricultural Research Technician

Gardner Hall 2317


Current supervisors: Prof. George Kennedy; Prof. Michael Reiskind

Position Responsibilities and Experience: 

    • Using bioinformatics tools and pipelines for analyzing high-throughput data in the context of population genetics research for the thrips species Frankliniella fusca and mosquito species Aedes aegypti and Aedes albopictus. Writing reports of results and presenting interpretation
    • Performing large genetic datasets manipulation: FastQC, Trimmomatic, SAMtools, VCFTools and SED regular expression language. Some knowledge of AWK command line. Unix bash scripting adapted to bioinformatics pipeline automation and customization
    • Performing de-novo and referenced SNPs discovery in the context of population genetics of insects using STACKS and PyRad
    • Performing population structure analysis using Genepop, Structure, Plink, fastStructure and Arlequin
    • Performing population migration inference by coalescence with Migrate software.
    • Performing sequences alignments using Bowtie2 and BWA
    • Performing phylogenic analyses using Mega and Phylip
    • Performing DAPC and PCA using Adegenet library and Genesis PCA and Admixture Plot Viewer
    • Performing Landscape genetics analysis using Geneland in R
    • Utilizing Geneious and CLC Workbench software for sequences alignments, microsatellites scoring and primer design
    • Applying knowledge of R programming language related to R-libraries requiring code customization
    • Application of TaqMan probes for TaqMan SNP Genotyping Assays on Spider mites Tetranychus urticae
    • Implementing 5Dye-sets microsatellite protocols for genotyping Tetranychus urticae samples on ABI 3730xl
    • Performing whole genome amplification technique (Qiagen Repli-G) on Frankliniella fusca samples in preparation for Nextera library preps in population genetics study
    • Performing DNA extractions on magnetic beads for NovaSeq 6000 library prep
    • Rearing clonal pure lines of thrips Frankliniella fusca and conducting subsequent sample dissections for draft genome assembly project
    • Performing screening of mosquito samples as potential carriers for dog heartworm Dirofilaria immitis using SYBRGreen qPCR method on mitochondrial amplicons
    • Performing insect DNA extractions (ethanol precipitation, phenol-chloroform, Chelex, column membrane, agar excision, CTAB) and DNA purification (Qiagen DNA clean-up kits, Zymo mercaptoethanol protocols)
    • Performing ddRad-Seq library prep for population studies
    • Genotyping of insect samples for population characterization using PCR amplification of microsatellites
    • Identification of insecticide resistance traits (KDR gene) in bedbugs using mitochondrial markers.
    • Insect species identification using mitochondrial barcodes (CO2, CO1, 16S) matched to GeneBank NCBI repository for a termite species Coptotermes formosanus study
    • Setup and operation of Li-Cor 4300 and LiCor 4200 DNA Analyzers
    • Gel electrophoresis of amplicons, gel excisions and utilization of BigDye Terminator v.3.1 kit sequencing prep
    • Population genetics statistical analysis of high-throughput data: F-Statistics, Genepop, Structure, Plink
    • Phylogenetic analysis of mitochondrial amplicons on VectorNTI, CLC Workbench, Sequencher, BioEdit, and Mega
    • Microsatellites development for the termite species Silvestritermes euamignathus and Heterotermes aureus (unpublished) on 454-GSFlx platform with subsequent assembly using bioinformatics tools (Velvet, Mira, SPAdes)
    • RNA isolation and sequencing for an RNA-seq project on Reticulitermes flavipes reproductive stages (unpublished)
    • Custom Taq enzyme production with TaQ plasmid transformation method of DH5a competent cells
    • Lab teaching helper for graduate course: Special Topics – Molecular Ecology ENT 495/591
    • Genetic data analysis of submitted samples and report write-ups as part of agrobusiness industry contracts (Dow, Syngenta)
    • Insecticides efficacy trials for agrochemical industry (BASF, Bayer, Syngenta): Setup and implementation of laboratory experiments and field bioassays
    • Preparation and titration of laboratory stock solutions
    • Phylogenetic analysis of haplotypes of field collected termite specimens of Coptotermes formosanus on a coastal China transect. GPS locations processed with ESRI GIS software
    • Laboratory rearing of subterranean termites Reticulitermes virginicus and flavipes from NC forests.
    • Supervision of temporary laboratory personnel
    • Routinely performing laboratory management duties: upkeep of chemical inventories, consumable supplies, equipment maintenance and drafting of safety plans for multiple laboratories


Hopperstad, K.A., Reiskind, M.H., Labadie, P.E. et al. Patterns of genetic divergence among populations of Aedes aegypti L. (Diptera: Culicidae) in the southeastern USA. Parasites Vectors 12, 511 (2019).

Burford Reiskind, MO, Labadie, P, Bargielowski, I, Lounibos, LP, Reiskind, MH. Rapid evolution and the genomic consequences of selection against interspecific mating. Mol Ecol. 2018; 27: 3641– 3654.

Jones, S. C., Vargo, E. L., Keefer, T. C., Labadie, P., Scherer, C. W., Gallagher, N. T., & Gold, R. E. (2017). Efficacy of chlorantraniliprole in controlling structural infestations of the eastern subterranean termite in the USA. Insects, 8(3), 92. doi:

Burford Reiskind, M.O., Coyle, K., Daniels, H.V., Labadie, P., Reiskind, M.H., Roberts, N.B., Roberts, R.B., Schaff, J. and Vargo, E.L. (2016), Development of a universal double‐digest RAD sequencing approach for a group of nonmodel, ecologically and economically important insect and fish taxa. Mol Ecol Resour, 16: 1303-1314.

Haifig, I., Vargo, E. L., Labadie, P., & Costa-Leonardo, A. (2016). Unrelated secondary reproductives in the neotropical termite silvestritermes euamignathus (isoptera: Termitidae). Die Naturwissenschaften, 103(1-2), 9. doi:

Perdereau, E., Bagnères, A.‐G., Vargo, E., Baudouin, G., Xu, Y., Labadie, P., Dupont, S. and Dedeine, F. (2015), Relationship between invasion success and colony breeding structure in a subterranean termite. Mol Ecol, 24: 2125-2142.

Vargo EL, Labadie PE, Matsuura K. Asexual queen succession in the subterranean termite Reticulitermes virginicus. Proc Biol Sci. 2012;279(1729):813–819.

R.J. Brightwell, P.E. Labadie, J. Silverman, Northward Expansion of the Invasive Linepithema humile (Hymenoptera: Formicidae) in the Eastern United States is Constrained by Winter Soil Temperatures, Environmental Entomology, Volume 39, Issue 5, 1 October 2010, Pages 1659–1665,

Matsuura, K., Vargo, E.L., Kawatsu, K., Labadie, P.E., Nakano, H., Yashiro, T., and Tsuji K. 2009. Queen succession through asexual reproduction in termites. Science 323:1687.


View Curriculum Vitae


Master, Biology, University Of Florence, Italy (2000)