Research Interests

My research focuses on the ecology of harmful algae, with special concentration on harmful cyanobacteria in North Carolina drinking waters and estuarine phytoplankton in the Neuse River Estuary.  I have more than a decade of experience in the isolation and culture of freshwater, estuarine, and marine microalgae, and the taxonomic evaluation of phytoplankton using light, scanning electron and transmission electron microscopy.  Other recent research involves mass culture and techniques development for maximizing lipid synthesis in the marine microalga Dunaliella for sustainable biofuel production.

Selected Publications

Wang, W.-C., E. Allen, A.A. Campos, R.K. Cade, L. Dean, M. Dvora, J.G. Immer , S. Mixson, S. Srirangan, M.-L. Sauer, S. Schreck, K. Sun, N. Thapaliya, C. Wilson, J. Burkholder, A.M. Grunden, H.H. Lamb, H. Sederoff, L.F. Stikeleather, and W.L. Roberts (2013) ASI: Dunaliella marine microalgae to drop-in replacement liquid transportation fuel. Environmental Progress (in press).

Burkholder, J.M., E.H. Allen, and C.A. Kinder (2010) Assessment of Water Resources and Watershed Conditions in Ocmulgee National Monument, Georgia. Natural Resource Report NPS/SECN/NRR—2010/276. National Park Service, Fort Collins, CO, 81 pp.

Burkholder, J.M., E.H. Allen, C.A. Kinder, and E.B. Morris (2010) Assessment of Water Resources and Watershed Conditions in the Chattahoochee River National Recreation Area, Georgia. Natural Resource Report NPS/SECN.NRR-2010/274. National Park Service, Fort Collins, CO.

Reed, R.E., J.M. Burkholder, and E.H. Allen (2010) Current online monitoring technology for surveillance of algal blooms, potential toxicity, and physical/chemical structure in rivers, reservoirs, and lakes, pp. 3-24. In: Algae Manual, AWWA Manual 57, by the American Water Works Association, Denver, CO.

Burkholder, J.M., G.M. Hallegraeff, G. Melia, A. Cohen, H.A. Bowers, D.W. Oldach, M.W. Parrow, M.J. Sullivan, P.V. Zimba, E.H. Allen, and M.A. Mallin (2007) Phytoplankton and bacterial assemblages in ballast water of U.S. military ships as a function of port of origin, voyage time and ocean exchange practices. Harmful Algae 6: 486-518.

Touchette, B.W., J.M. Burkholder, E.H. Allen, J.L. Alexander, C.A. Kinder, J. James, and C.H. Britton (2007) Eutrophication and cyanobacteria blooms in run-of-river impoundments in North Carolina, U.S.A. Lake and Reservoir Management 23: 179-192.

Marshall, H.G., P.E. Hargraves , J.M. Burkholder, M.W. Parrow, M. Elbrächter, E.H. Allen, V.A. Knowlton, P.A. Rublee, W.L. Hynes, T.A. Egerton, D.L. Remington, K.B. Wyatt, A.J. Lewitus, and V.C. Henrich VC (2006) Taxonomy of Pfiesteria (Dinophyceae). Harmful Algae 5: 481-496.

Parrow, M.W., M. Elbrächter, M.K. Krause, J.M. Burkholder, N.J. Deamer, N. Hyte, and E.H. Allen (2006) The taxonomy and growth of a Crypthecodinium species (Dinophyceae) isolated from a brackish water fish aquarium. African Journal of Marine Science 28: 185-191.

Zimba, P.V., A. Camus , K. Gregg, E.H. Allen, and J.M. Burkholder (2006) Co-occurrence of white shrimp, Penaeus vannamei, mortalities and microcystin toxin in a southeastern USA shrimp facility. Aquaculture 261: 1048-1055.

Zhang, C., E.H. Allen, H.B. Glasgow, P.D.R. Moeller, J.M. Burkholder, A.J. Lewitus, G.M. Melia, and S.L. Morton (2004) Evaluation of toxicity in nine raphidophyte strains isolated from different geographic regions, pp. 198-200. In: Harmful Algae 2002 – Proceedings of the Xth International Conference on Harmful Algae, by Steidinger KA, Landsberg JA, Tomas CR, Vargo GA (eds.). Florida Fish and Wildlife Conservation Commission, Florida Institute of Oceanography, and the IOC of UNESCO, St. Petersburg, FL.