Dominique "Niki" Robertson - Professor
Virus-induced gene silencing, geminivirus resistance
My lab has active areas of research in virus-induced gene silencing, geminivirus-host interactions, and in abiotic stress responses. Long-term goals are to understand how geminiviruses, which are DNA viruses that transcribe their genes in plant nuclei, interact with plant gene silencing pathways. We are currently mapping an Arabidopsis locus that confers resistance to two different genera of the Geminiviridae, begomoviruses and curtoviruses.
Hanley-Bowdoin, L., Bejarano, E.R., Robertson, D., Mansoor, S. (2013) Geminiviruses: Masters at redirecting and reprogramming plant processes. Nature Reviews: Microbiology. 11, 777-788.
Robertson, D. (2013) Modulating plant calcium for better nutrition and stress tolerance. ISRN Botany 2013:1-22. (Invited review) http://dx.doi.org/10.1155/2013/952043
Tuttle, J.R., Haigler, C.H., Robertson, D. (2012) Method: Low-cost delivery of the Cotton leaf crumple virus-induced gene silencing system. Plant Methods 8:27-35.
Bernacki, S., Karimi, M., Hilson, P., Robertson, D. (2010) Viral-Induced Gene Silencing as a Reverse Genetics Tool to Study Gene Function. In: Methods in Molecular Biology: Plant Developmental Biology. Eds: Lars Hennig Totowa, NJ: Humana.
Tuttle. J.R., Idris, A.M., Brown, J.K., Haigler, C.H., Robertson, D. (2008) Geminivirus-mediated Gene Silencing from Cotton Leaf Crumple Virus Is Enhanced by Low Temperature in Gossypium hirsutum. Plant Physiol 148: 41-50.
Jordan, C.V., Shen, W., Hanley-Bowdoin, L., and Robertson, D. (2007) Geminivirus-induced gene silencing of the tobacco retinoblastoma-related gene results in cell death and altered development. Plant Molecular Biology. 65: 163-175.
Blevins, T., Rajeswaran, R., Shivaprasad, P.V., Beknazariants, D., Si-Ammour, A., Park, H.-S. Vazquez, F. Robertson, D., Meins, F., Hohn, T. and Pooggin, P., 2006 Four plant Dicers mediate viral small RNA biogenesis and DNA virus induced silencing. Nucleic Acids Research 34:6233-6246.
Muangsan, N., Beclin, C., Vaucheret, H., Robertson, D. (2004) Geminivirus VIGS of endogenous genes requires SGS2/SDE1 and SGS3 and defines a new branch in the genetic pathway for silencing in plants. Plant J. 38: 1004-1014.
Robertson, D. (2004) VIGS vectors for gene silencing, many targets, many tools. Ann. Rev. Plant Biol. 55: 495-519.
Nagar, S., Hanley-Bowdoin, L., and Robertson, D. (2002) Host DNA Synthesis is Induced by Geminivirus Infection of Differentiated Plant Cells. The Plant Cell. 14:2995-3007.
Turnage, M.A., Muangsan, N., Peele, C.G, and Robertson, D. (2002) Geminivirus-based vector for gene silencing in Arabidopsis. Plant J. 30: 107-114.
Peele, C., Jordan, C.V., Muangsan, N., Turnage, M., Egelkrout, E., Eagle, P., Hanley-Bowdoin, L., and Robertson, D. (2001) Silencing of a meristematic gene using geminivirus-derived vectors. Plant J. 27:357-66
Kjemtrup, S., Sampson, K.S., Peele, C., Nguyen, L.V., Conkling, M.A., Thompson, W.F. and Robertson, D. (1998) Gene silencing from plant DNA carried by a geminivirus. The Plant Journal, 14: 91-100.
Nagar, S. Pedersen, T., Carrick, K., Hanley-Bowdoin, L. and Robertson, D.
(1995) A geminivirus induces expression of a host DNA synthesis protein in
terminally differentiated plant cells. The Plant Cell 7: 705-719.